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KMID : 0380020190340030185
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 3 p.185 ~ p.191
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Apoptotic Effects of Parthenocissus tricuspidata (Siebold & Zucc.) Planch Ethanol Extract by regulating AMPK/Akt/mTOR Signaling Pathways in A549 Cancer Cells
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Jo Kyung-Jo
Nam Gun-He
Park Ye-Seul
Kim Sang-Yong
Koo Bong-Seong
Kim Young-Min
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Abstract
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Lung cancer can be caused by several environmental factors, such as smoking and urban and industrial pollution. Lung cancer also has a higher recurrence rate and a lower cure rate than other types of cancer. Parthenocissus tricuspidata (Siebold & Zucc.) Planch Extract (PTE) is known to have antioxidant, antidiabetic, and anti-inflammatory effects; however, its efficacy as an anticancer agent has not been reported. The purpose of this study was to investigate the effect of PTE on apoptosis-related proteins in A549 lung cancer cells and to determine how apoptosis is induced through the AMP-activated protein kinase (AMPK)/Akt/mTOR signaling pathway. AMPK is activated by metabolic stress, such as glucose deficiency, and induces apoptosis through the intrinsic pathway that activates tumor suppressor p53, thereby regulating the bcl-2 family protein, releasing cytochrome c from the mitochondria, and activating caspase. In addition, Akt plays an important role in the metabolism, growth, and survival of cancer cells. Inhibition of Akt induces apoptosis by regulating key signaling molecules, such as the mammalian target of rapamycin and tumor suppressor p53. In this study, we examined the effect of PTE on the apoptosis of A549 cells and determined how the regulation of apoptosis proteins through the intrinsic pathway is achieved. Our results demonstrated that PTE regulates bcl-2 proteins and induces apoptosis through the intrinsic pathway. In addition, AMPK and Akt inhibitors were treated to determine whether these apoptotic effects were dependent on AMPK and Akt.
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KEYWORD
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A549, apoptosis, Parthenocissus tricuspidata (Siebold & Zucc.) Planch, anti-cancer, AMPK/Akt/mTOR pathway, Intrinsic pathway, Bcl-2 family
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